A cell line is a population of cells derived from a few cells of a multicellular organism, generated in vitro from primary culture after the first passage. The cell line contains genetic information identical to the organism from which it was derived. Cell lines derived from normal (non-cancerous) cells and tissues have a limited ability for division and can be maintained in culture for a limited time. The number of passages of cell line depends on the type of tissue and age of the organism from which the cell line was derived, and the limited number of cell divisions reflects in vivo aging of cells. Cell lines derived from stem cells (embryonic, adult or induced) or immortalised cells, evading cellular senescence due to a mutation, live longer. In comparison to animal models, cell lines are more cost effective, easy to maintain, able to provide more reproducible results, and usually (with the exception of embryonic cells) burdened by fewer ethical concerns, which makes them an attractive material for biological and medical research. However, it is necessary to remember that being derived from a small number of cells, and at the same time susceptible to genetic changes over the time of extended culture and number of passages, cell lines may not always adequately represent primary cell populations.
Cell lines with an unlimited life-span and the ability to divide infinitely can be derived from cancerous tumors.
Wersja z 15:50, 21 sty 2021
Definition
A cell line is a population of cells derived from a few cells of a multicellular organism, generated in vitro from primary culture after the first passage. The cell line contains genetic information identical to the organism from which it was derived. Cell lines derived from normal (non-cancerous) cells and tissues have a limited ability for division and can be maintained in culture for a limited time. The number of passages of cell line depends on the type of tissue and age of the organism from which the cell line was derived, and the limited number of cell divisions reflects in vivo aging of cells. Cell lines derived from stem cells (embryonic, adult or induced) or immortalised cells, evading cellular senescence due to a mutation, live longer. In comparison to animal models, cell lines are more cost effective, easy to maintain, able to provide more reproducible results, and usually (with the exception of embryonic cells) burdened by fewer ethical concerns, which makes them an attractive material for biological and medical research. However, it is necessary to remember that being derived from a small number of cells, and at the same time susceptible to genetic changes over the time of extended culture and number of passages, cell lines may not always adequately represent primary cell populations.
Cell lines with an unlimited life-span and the ability to divide infinitely can be derived from cancerous tumors.