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=Immortalised cell lines= | =Immortalised cell lines= | ||
The mutations that cause the cell immortalisation can occur naturally – such is the case of cell lines derived from cancers, e.g. in the first human cell line, HeLa, or can be induced under laboratory conditions by chemical or | The mutations that cause the cell immortalisation can occur naturally – such is in the case of cell lines derived from cancers, e.g. in the first human cell line, HeLa, or can be induced under laboratory conditions by chemical or oncogene mediated transformation. In the latter case, this is achieved by overexpressing telomerase and TERT, inactivating cell cycle checkpoints p53 and pRb, or introducing oncogenes or viral vectors encoding oncoproteins, which also interfere with cell cycle regulators. There is, however, a risk of undesired carcinogenic effects when using immortalised cells.<ref>Paprocka M, Krawczenko A, Dus D, Kantor A, Carreau A, Grillon C, Kieda C. CD133 positive progenitor endothelial cell lines from human cord blood. Cytometry A. 2011 Aug;79(8):594-602. doi: 10.1002/cyto.a.21092. Epub 2011 Jun 27.</ref> <ref>Katsumiti A, Ruenraroengsak P, Cajaraville MP, Thorley AJ, Tetley TD. Immortalisation of primary human alveolar epithelial lung cells using a non-viral vector to study respiratory bioreactivity in vitro. Sci Rep. 2020 Nov 24;10(1):20486. doi: 10.1038/s41598-020-77191-y.</ref> | ||
Aktualna wersja na dzień 11:41, 15 kwi 2021
Immortalised cell lines
The mutations that cause the cell immortalisation can occur naturally – such is in the case of cell lines derived from cancers, e.g. in the first human cell line, HeLa, or can be induced under laboratory conditions by chemical or oncogene mediated transformation. In the latter case, this is achieved by overexpressing telomerase and TERT, inactivating cell cycle checkpoints p53 and pRb, or introducing oncogenes or viral vectors encoding oncoproteins, which also interfere with cell cycle regulators. There is, however, a risk of undesired carcinogenic effects when using immortalised cells.[1] [2]
- ↑ Paprocka M, Krawczenko A, Dus D, Kantor A, Carreau A, Grillon C, Kieda C. CD133 positive progenitor endothelial cell lines from human cord blood. Cytometry A. 2011 Aug;79(8):594-602. doi: 10.1002/cyto.a.21092. Epub 2011 Jun 27.
- ↑ Katsumiti A, Ruenraroengsak P, Cajaraville MP, Thorley AJ, Tetley TD. Immortalisation of primary human alveolar epithelial lung cells using a non-viral vector to study respiratory bioreactivity in vitro. Sci Rep. 2020 Nov 24;10(1):20486. doi: 10.1038/s41598-020-77191-y.